CHANGES IN THE CELLULAR IMMUNE PROTECTION OF ORGANISM IN THE PROCESS OF CHRONIZATION OF EXPERIMENTAL BACTERIAL-IMMUNE PERIODONTITIS

  • A.Ye. Demkovych I. Horbachevsky Ternopil State Medical University
  • Yu.I. Bondarenko I. Horbachevsky Ternopil State Medical University
  • M.M. Yakymchuk I. Horbachevsky Ternopil State Medical University
Keywords: bacterial-immune periodontitis, immune system, T-lymphocytes, B-lymphocytes, inflammation.

Abstract

One of the important factors that leads to damage of structures of the periodontal complex and leads to the formation of inflammatory process of varying degrees is the disruption of immunological processes.

The aim of the study was to clarify the pathogenetic role of cellular adaptive immunity in the process of formation of chronic inflammatory reaction in the late period of the experimental bacterial-immune periodontitis. The study was conducted on white, non-breeding, clinically healthy male rats. Experimental bacterial-immune periodontitis in experimental animals was caused by insertion into the tissues of the periodontal complex a mixture of microorganisms diluted with egg protein. The obtained digital data was statistically processed using parametric and nonparametric statistical methods.

The article represents the results of research on the parameters of cellular immune defense, determined by the relative number of CD3+ (common T-lymphocytes), CD4+ (T-helpers), CD8+ (cytotoxic cells, T-killers), CD19+ (B-lymphocytes), CD16+ (natural killers, NK-cell) and immunoregulatory index (CD4+ / CD8+) in intact animals and on the 30th day of experimental bacterial-immune periodontitis development. It was established that the nature of the course of experimental inflammation in the tissues of the periodontal complex depended on changes in the cellular immune status, accompanied by a decrease of the content of common mature T-lymphocytes (CD3+) in the blood of animals with experimental bacterial-immune periodontitis on the 30th day of the study. In the process of the development of the experimental bacterial-immune periodontitis there was a decrease of the content of T-lymphocytes-helper (CD4+) in the blood of animals and on the 30th day of the study an increase in T-suppressors (CD8+), an increase in the content of natural killers (CD16+) and a decrease in the relative content of B-lymphocytes (CD19+). The immunoregulatory index (CD4+ / СD8+) decreased in comparison with this indicator of a group of intact animals. In rats with bacterial-immune periodontitis, an immunosuppressive state developed in the late period of the inflammatory reaction due to both T-helper cells and cytotoxic T-suppressors / killers. These changes can be considered as signs of formation of the chronic course of the inflammatory process in the tissues of periodontal complex.

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Published
2018-03-21
How to Cite
Demkovych, A., Bondarenko, Y., & Yakymchuk, M. (2018). CHANGES IN THE CELLULAR IMMUNE PROTECTION OF ORGANISM IN THE PROCESS OF CHRONIZATION OF EXPERIMENTAL BACTERIAL-IMMUNE PERIODONTITIS. Ukrainian Dental Almanac, (1), 5-8. https://doi.org/10.31718/2409-0255.1.2018.01
Section
EXPERIMENTAL-THEORETICAL SECTION