THE STUDY OF BIOCHEMICAL INDIXES OF ORAL FLUID IN ORTHODONTIC TREATMENT

  • Yu.V. Filimonov National Pirogov Memorial Medical University, Vinnytsya, Ukraine
  • V.M. Istoshyn National Pirogov Memorial Medical University, Vinnytsya, Ukraine
  • A.V. Pachevska National Pirogov Memorial Medical University, Vinnytsya, Ukraine
  • N.V. Drachuk National Pirogov Memorial Medical University, Vinnytsya, Ukraine
  • O.P. Dudik National Pirogov Memorial Medical University, Vinnytsya, Ukraine
  • V.Yu. Filimonov National Pirogov Memorial Medical University, Vinnytsya, Ukraine
Keywords: maxillo-facial anomalies, orthodontic treatment, children, oral liquid, biochemical composition.

Abstract

The work is devoted to the study of the reactivity of the biochemical composition of saliva in children with SCI, which was used for the treatment of non-removable and removable OA. There was a decrease in the activity of the α-amylase enzyme during orthodontic treatment in both experimental groups: in patients in the experimental group, which used non-removable equipment 3 months after starting treatment at 16.5% and 6 months after starting treatment it was 16%. There was a decrease in the activity of saliva α-amylase after 3 months at 11% and in samples of saliva taken after 6 months at 10% during treatment of patients of the experimental group with removable orthodontic equipment. The total calcium level after 3 months of observation was 15% lower compared to the start of treatment. The phosphorus level was 9% lower at this moment of observation. After 6 months of observation, the trace elements were different from the original ones: calcium was 27% lower, and the level of phosphorus was lower comparing to the baseline by 15%. Treatment of SCI with removable orthodontic equipment impaired the mineral balance: the calcium level after 3 months of observation was lower by 12%, and the level of phosphorus was lower by 6% compared to the beginning of the study. After 6 months of observation, the traced elements differed from the original ones: the level of calcium was 21% lower than the previous values, and the level of phosphorus was lower by 13% compared to the start of treatment. The evaluation of alkaline phosphatase activity in mixed unstimulated saliva using non-removable orthodontic equipment showed that the level of alkaline phosphatase after 3 months of treatment was 3% lower compared to the beginning of treatment, and after 6 months of observation activity of the investigated enzyme was less than the initial by 10%. Treatment of dental anomalies with removable equipment inhibited the enzyme activity: after 3 months of treatment, the activity of alkaline phosphatase was less than 7% compared to the beginning of the study, and after 6 months of observation activity of the investigated enzyme differed from the initial by 20%. The use of non-removable orthodontic equipment led to a significant increase in the activity of IL-1β. Three months after starting the study, IL-1β was 4-fold higher compared to the start of treatment, and after 6 months of treatment, it became 9-fold higher. Treatment of dental abnormalities with removable equipment showed that the activity of IL-1β after 3 months of treatment increased 4-fold, and after 6 months of observation the researched index was different from the initial 9-fold.

At the 3rd month of observation in children using non-removable OA, the activity of lysozyme decreased by 21%; reduction of lysozyme activity during the 6th month of treatment constituted 23%. Patients in the experimental group treated with detachable OA observed an increase in lysozyme activity at the 3rd month of observation by 27%, and 6 months after the start of treatment, the activity of lysozyme was less than 29% compared to the data of the 3rd month of the use of non-removable orthodontic equipment it led to a significant increase in the level of anti-inflammatory cytokine: after 3 months of observation was 2.5 times more than at the beginning of treatment, and after 6 months of observation, they differed from the initial in 4 times. Treatment of dental anomalies with removable orthodontic equipment also contributed to an increase in anti-inflammatory cytokine: IL-4 levels were higher after 3 months of treatment compared to baseline data by 1,8, and after 6 months of follow-up, the study figure was in 4 times higher than the previous values.

The obtained results of the study of the reactivity of biochemical changes in oral fluid using removable and non-removable OA in children can be used in planning of orthodontic treatment as diagnostic markers for the further selection of tactics and assessment of the oral cavity in children on the background of treatment of JFA by removable and non-removable OA.

Downloads

Download data is not yet available.

References

1. Безвушко Е. В. Порівняльна оцінка стоматологічного здоров’я дітей шкільного віку за Європейськими індикаторами здоров’я порожнини рота / Е. В. Безвушко, Л. Ф. Жугіна, А. А. Нарикова, Н. Л. Чухрай // Новини стоматології. — 2014. – №3. — С. 76–78.

2. Бутюгин И. А. Состояние системы перекисного окисления липидов–антиоксидантная защита в смешанной слюне у больных хроническим генерализованным пародонтитом/ И. А. Бутюгин, И. А. Волчегорский // Клиническая лабораторная диагностика. — 2014. — № 2. — С. 44–47.

3. Волинская Т. Б. Дифференциальный подход в выборе порошка для абразивной обработки зубов при лечении больных с воспалительными заболеваниями тканей пародонта / Т. Б. Волинская // Стоматология. — 2013. — №2. — С. 27–30.

4. Воронкова А. В. Лечебно–профилактическое действие симбиотика «Симбитер» при экспериментальном стоматите / А. В. Воронкова, Л. В. Смаглюк, А. П. Левицкий // Український стоматологічний альманах. — 2012. — №1.

5. Головко Н.В. Ортодонтия / Н. В. Головко. — Вінниця: Нова книга, 2008. — 220 с.

6. Доменюк Д. А. Взаимосвязь гематологических показателей кальций–фосфорного обмена с параметрами метаболизма в ротовой жидкости у пациентов с зубочелюстной патологией / Д. А. Доменюк // Кубанский научный медицинский вестник. — 2015. — №1(150). — С. 54–65.

7. Дорофейчук В. Г. Определение активности лизоцима нефелометрическим методом / В. Г. Дорофейчук // Лабораторное дело. — 1968. — №1. — С. 28–30.

8. Іванов В. С. Стоматологічна захворюваність у дітей дошкільного та молодшого шкільного віку міста Одеса (частина 1) / В. С.Іванов // Вісник стоматології. — 2013. — №1. — С. 121–125.

9. Калініченко Ю. А. Взаємозв’язок та взаємовідносини стоматологічного та соматичного здоров’я дітей та підлітків як сучасна медико–соціальна проблема / Ю. А. Калініченко, Т. А. Сіротченко // Здоровье ребенка. — 2010. — №3 (24).

10. Клітинська О. В. Аналіз біохімічних параметрів слини у дошкільнят з некомпенсованою формою множинного карієсу, які постійно проживають в умовах біогеохімічного дефіциту фтору та йоду / О. В. Клітинська // Вісник проблем біології і медицини. — 2015. — Вип. 2, т. 4(121). — С. 309–312.

11. Лабунец В. А. Состояние и тенденции развития стоматологической ортопедической помощи у лиц молодого возраста / В. А. Лабунець // Український стоматологічний альманах. — 2013. — №5. — С. 85–88.

12. Манжуловская В. В. Самое современное в ортодонтии — «невидимые брекеты» / В. В. Манжуловская // Современная стоматология. — 2013. — №3. — С. 143–145.

13. Оспанова Г. Б. Непосредственные и отдаленные результаты ортодонтического лечения взрослых / Г. Б. Оспанова // Стоматология. — 1978. — №2. — С. 61–63.

14. Савинчук Н. О. Аналіз програм профілактики основних стоматологічних захворювань у розвинених країнах / Н. О. Савинчук, О. В. Клітинська // Современная стоматология. — 2014. — №4. — С. 64–66.

15. Смаглюк Л. В. Применение схемы индивидуальной профилактической программы при лечении брекет–техникой / Л. В. Смаглюк, Е. В. Лучко, С. В. Давыденко, А. Омар // Український стоматологічний альманах. — 2013. — №3. — С. 65–67.

16. Фаустова М. А. Изменение активности лизоцима ротовой жидкости при дентальной имплантации / М. А. Фаустова, О. В. Добровольская, А. В. Добровольский // Стоматологическая наука и практика. — 2015. –№ 3–4(8–9). —С. 22–25.

17. Euro Barometer 72.3 Report. Oral Health, TNS, Brussels. — 2010. — 90 p.

18. Maes L. Tooth brushing in 32 countries / L. Maes // International Dental Journal.— 2006. —Vоl. 56. — P. 159–160.
Published
2018-06-25
How to Cite
Filimonov, Y., Istoshyn, V., Pachevska, A., Drachuk, N., Dudik, O., & Filimonov, V. (2018). THE STUDY OF BIOCHEMICAL INDIXES OF ORAL FLUID IN ORTHODONTIC TREATMENT. Ukrainian Dental Almanac, (2), 21-26. https://doi.org/10.31718/2409-0255.2.2018.05
Section
ORTHODONTICS